Details

Autor(en) / Beteiligte

• Amoozegar, M. A
• Malekzadeh, F
• Malik, K. A
• Schumann, P
• Sproer, C

Titel

Halobacillus karajensis sp. nov., a novel moderate halophile

Ist Teil von

• International journal of systematic and evolutionary microbiology, 2003-07, Vol.53 (4), p.1059-1063

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• 1 Department of Biology (Microbiology Unit), Faculty of Science, University of Tehran, Tehran, Iran 2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany Correspondence F. Malekzadeh falmero{at}yahoo.com A moderately halophilic, Gram-positive, spore-forming bacterium was isolated from surface saline soil of the Karaj region, Iran. The strain, designated MA-2 T , was strictly aerobic with rod-shaped cells that occurred singly, in pairs or short chains. It contained L -orn– D -Asp-type peptidoglycan and the major respiratory lipoquinone was MK-7. It was non-motile and had an ellipsoidal endospore located centrally or subterminally. Growth occurred at 10–49 °C and in the pH range 6·0–9·6. Strain MA-2 T grew at salinities of 1–24 % (w/v) NaCl, showing optimal growth at 10 % (w/v). The DNA G+C content was 41·3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MA-2 T was associated with Bacillus rRNA group 1. The micro-organisms showing the closest phylogenetic relationship to strain MA-2 T were Halobacillus litoralis and Halobacillus trueperi . On the basis of phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA–DNA similarity data, it is proposed that strain MA-2 T (=DSM 14948 T =LMG 21515 T ) should be placed in the genus Halobacillus as the type strain of a novel species, Halobacillus karajensis sp. nov. The EMBL accession number for the 16S rDNA sequence of strain MA-2 T (=DSM 14948 T ) is AJ486874 .