Details

Autor(en) / Beteiligte

• Nahorski, Stefan R.
• Young, Kenneth W.
• John Challiss, R.A.
• Nash, Mark S.

Titel

Visualizing phosphoinositide signalling in single neurons gets a green light

Ist Teil von

• Trends in neurosciences (Regular ed.), 2003-08, Vol.26 (8), p.444-452

Ort / Verlag

Oxford: Elsevier Ltd

Erscheinungsjahr

2003

Quelle

ScienceDirect

Beschreibungen/Notizen

• There is now substantial evidence, from single-cell imaging, that complex patterns of release from Ca 2+ stores play an important role in regulating synaptic efficacy and plasticity. Moreover, the major mechanism of store release depends on the generation of inositol 1,4,5-trisphosphate [Ins(1,4,5) P 3] through the action of phospholipase(s) C on phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5) P 2], and several neurotransmitters can enhance receptor-mediated activation of this enzyme. The recent development of techniques to image real-time changes in PtdIns(4,5) P 2 hydrolysis according to generation of Ins(1,4,5) P 3 and diacylglycerol in single cells has significantly advanced our ability to investigate these signalling pathways, particularly in relation to single-cell Ca 2+ signals. This article reviews these new approaches and how they have provided novel insights into mechanisms underlying spatio-temporal Ca 2+ signals and phospholipase C activation in neurons.