Details

Autor(en) / Beteiligte

• Müller, Linda J.
• Cardozo, Bob Nunes
• Vrensen, Gijs F. J. M.

Titel

Quantitative aspects of synapses on Golgi-impregnated neurons

Ist Teil von

• Microscopy research and technique, 1992-12, Vol.23 (4), p.334-352

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

1992

Quelle

Access via Wiley Online Library

Beschreibungen/Notizen

• With the classical Golgi techniques, numerous types of neurons can be distinguished in the cerebral cortex, each with a specific dendritic geometry and pattern of axonal ramifications. In the present review we describe two techniques which allow quantification of synapses on identified neurons: (1) Golgi‐rapid impregnation‐gold toning‐electron microscopy, and (2) Golgi‐Kopsch impregnation‐gold toning‐electron microscopy in combination with staining of the tissue with ethanolic phosphotungstic acid (E‐PTA). Both techniques were applied on neurons in the visual cortex of young and adult rabbits. By means of rotating and tilting specimens in the electron microscope, the nondistinctive ultrastructure of obliquely sectioned synapses can be circumvented, leading to precise estimates of asymmetrical vs. symmetrical synapses without complete reconstruction of the neuron. © 1992 Wiley‐Liss, Inc.