Details

Autor(en) / Beteiligte

• Monser, Lotfi
• Darghouth, Frida

Titel

Simultaneous determination of naproxen and related compounds by HPLC using porous graphitic carbon column

Ist Teil von

• Journal of pharmaceutical and biomedical analysis, 2003-08, Vol.32 (4), p.1087-1092

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• A simple, selective and sensitive high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of naproxen and its main degradation products such as 1-(6-methoxy-2-naphthyl) ethanol (MNE), 2-methoxy-6-ethyl naphthalene (MEN) and 2-acetyl-6-methoxy naphthalene (AMN). The separation of these compounds was achieved on porous graphitic carbon (PGC) column using tetrahydrofuran–methanol as the mobile phase, and the effluent from the column was monitored at 272 nm. At a flow rate of 1 ml min −1, the retention time of the last eluting compound was less than 10 min. Correlation coefficient for calibration curves in the ranges 2–25 μg ml −1 for all compounds studied were greater than 0.999. The sensitivity of detection is 0.05 μg l −1 for naproxen, MNE and MEN and 0.20 μg ml −1 for AMN. The reproducibility of the peak area of these compounds using isocratic elution were quite high, and the standard deviations (S.D.) were below 2% ( n=5). The reproducibility of retention times of these compounds was within 1% ( n=5). The proposed liquid chromatographic method was successfully applied to the analysis of commercially available naproxen sodium (NS) dosage forms with recoveries of 98.8–102%. A comparative study shows that the selectivity of these compounds on PGC column was different to that obtained with octadecyl silica (ODS) columns.