Analytical biochemistry, 1992-12, Vol.207 (2), p.231-235
1992
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- High resolution separation and quantitation of ribonucleotides using capillary electrophoresis
- Ist Teil von
- Analytical biochemistry, 1992-12, Vol.207 (2), p.231-235
- Ort / Verlag
- San Diego, CA: Elsevier Inc
- Erscheinungsjahr
- 1992
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- A method for the analysis of ribonucleotides using capillary electrophoresis has been developed. A cross-linked polyacrylamide coated column, Tris-HCl, and phosphate-mixed buffer were used, which produced reproducible separations (solute migration time average RSD% of 1.2) of 14 ribonucleotides within 50 min. Linear relationships between peak areas and sample concentrations, an average minimum detectable concentration of 5.4 μ m, and an average minimum detectable quantity of 0.08 pmol were obtained. The described method allowed reproducible and reliable qualitative and quantitative analysis of intracellular ribonucleotide pools in HeLa cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0003-2697eISSN: 1096-0309DOI: 10.1016/0003-2697(92)90005-RTitel-ID: cdi_proquest_miscellaneous_73437216
- Format
- –
- Schlagworte
- Analytical, structural and metabolic biochemistry, Biological and medical sciences, Capillary Action, Electrophoresis, Polyacrylamide Gel - methods, Fundamental and applied biological sciences. Psychology, HeLa Cells, Humans, Microchemistry - methods, Nucleic acids, Nucleic bases, nucleotides, Ribonucleotides - analysis, Ribonucleotides - isolation & purification