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BibTeX
Dermal fibroblast-mediated BMP2 therapy to accelerate bone healing in an equine osteotomy model
Journal of orthopaedic research, 2010-03, Vol.28 (3), p.403-411
Ishihara, Akikazu
Zekas, Lisa J.
Litsky, Alan S.
Weisbrode, Steven E.
Bertone, Alicia L.
2010
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Ishihara, Akikazu
Zekas, Lisa J.
Litsky, Alan S.
Weisbrode, Steven E.
Bertone, Alicia L.
Titel
Dermal fibroblast-mediated BMP2 therapy to accelerate bone healing in an equine osteotomy model
Ist Teil von
Journal of orthopaedic research, 2010-03, Vol.28 (3), p.403-411
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2010
Quelle
MEDLINE
Beschreibungen/Notizen
This study evaluated healing of equine metacarpal/metatarsal osteotomies in response to percutaneous injection of autologous dermal fibroblasts (DFbs) genetically engineered to secrete bone morphogenetic protein‐2 (BMP2) or demonstrate green fluorescent protein (GFP) gene expression administered 14 days after surgery. Radiographic assessment of bone formation indicated greater and earlier healing of bone defects treated with DFb with BMP2 gene augmentation. Quantitative computed tomography and biomechanical testing revealed greater mineralized callus and torsional strength of DFb‐BMP2‐treated bone defects. On the histologic evaluation, the bone defects with DFb‐BMP2 implantation had greater formation of mature cartilage and bone nodules within the osteotomy gap and greater mineralization activity on osteotomy edges. Autologous DFbs were successfully isolated in high numbers by a skin biopsy, rapidly expanded without fastidious culture techniques, permissive to adenoviral vectors, and efficient at in vitro BMP2 protein production and BMP2‐induced osteogenic differentiation. This study demonstrated an efficacy and feasibility of DFb‐mediated BMP2 therapy to accelerate the healing of osteotomies. Skin cell‐mediated BMP2 therapy may be considered as a potential treatment for various types of fractures and bone defects. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:403–411, 2010
Sprache
Englisch
Identifikatoren
ISSN: 0736-0266
eISSN: 1554-527X
DOI: 10.1002/jor.20978
Titel-ID: cdi_proquest_miscellaneous_734239723
Format
–
Schlagworte
adenovirus
,
Animals
,
BMP2
,
Bone and Bones - physiopathology
,
bone healing
,
Bone Morphogenetic Protein 2 - administration & dosage
,
Bone Morphogenetic Protein 2 - genetics
,
Bone Morphogenetic Protein 2 - secretion
,
Cell Transplantation - methods
,
dermal fibroblast
,
Dermis - cytology
,
Feasibility Studies
,
Fibroblasts - secretion
,
Fibroblasts - transplantation
,
Genetic Engineering
,
Horses
,
Injections
,
Metacarpal Bones - surgery
,
Metatarsal Bones - surgery
,
Osteotomy
,
Transduction, Genetic
,
Wound Healing
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