Details

Autor(en) / Beteiligte

• Rivera, Rebecca
• Nollens, Hendrik H
• Venn-Watson, Stephanie
• Gulland, Frances M. D
• Wellehan, James F. X., Jr

Titel

Characterization of phylogenetically diverse astroviruses of marine mammals

Ist Teil von

• Journal of general virology, 2010-01, Vol.91 (1), p.166-173

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

2010

Link zum Volltext

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• 1 Hubbs–SeaWorld Research Institute, San Diego, CA 92109, USA 2 Marine Mammal Health Program, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA 3 Navy Marine Mammal Program Foundation, San Diego, CA 92106, USA 4 The Marine Mammal Center, 2000 Bunker Road, Sausalito, CA 94965, USA Correspondence Rebecca Rivera Rrivera{at}hswri.org Astroviruses are small, non-enveloped, positive-stranded RNA viruses. Previously studied mammalian astroviruses have been associated with diarrhoeal disease. Knowledge of astrovirus diversity is very limited, with only six officially recognized astrovirus species from mammalian hosts and, in addition, one human and some bat astroviruses were recently described. We used consensus PCR techniques for initial identification of five astroviruses of marine mammals: three from California sea lions ( Zalophus californianus ), one from a Steller sea lion ( Eumetopias jubatus ) and one from a bottlenose dolphin ( Tursiops truncatus ). Bayesian and maximum-likelihood phylogenetic analysis found that these viruses showed significant diversity at a level consistent with novel species. Astroviruses that we identified from marine mammals were found across the mamastrovirus tree and did not form a monophyletic group. Recombination analysis found that a recombination event may have occurred between a human and a California sea lion astrovirus, suggesting that both lineages may have been capable of infecting the same host at one point. The diversity found amongst marine mammal astroviruses and their similarity to terrestrial astroviruses suggests that the marine environment plays an important role in astrovirus ecology. The GenBank/EMBL/DDBJ accession numbers for the partial capsid gene and full-length RdRp gene sequences of novel astroviruses CslAstV-1, -2, -3, SslAstV-1 and BdAstV-1 are FJ890351 [GenBank] –FJ890355 [GenBank] , respectively. A supplementary table showing the primers used and supplementary figures showing RdRp and capsid alignments are available with the online version of this paper.