Molecular and cellular probes, 1992-10, Vol.6 (5), p.361-365
1992
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Details
- Autor(en) / Beteiligte
- Titel
- An alternative to DNA extraction for the diagnosis of Pneumocystis carinii pneumonia by polymerase chain reaction using a new oligonucleotide probe
- Ist Teil von
- Molecular and cellular probes, 1992-10, Vol.6 (5), p.361-365
- Ort / Verlag
- Kidlington: Elsevier Ltd
- Erscheinungsjahr
- 1992
- Quelle
- Elsevier ScienceDirect Journals
- Beschreibungen/Notizen
- We have used new specific primers and probe in a polymerase chain reaction (PCR) followed by Southern blot assays to detect Pneumocystis carinii in human bronchoalveolar lavage samples obtained from HIV-infected patients with pulmonary symptoms. To facilitate the procedure we developed a filtration technique without DNA extraction yielding a high sensitivity ( 18 18 positive results). The high specificity of the technique was shown by testing immunosuppressed patients without P. carinii pneumonia.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0890-8508eISSN: 1096-1194DOI: 10.1016/0890-8508(92)90028-VTitel-ID: cdi_proquest_miscellaneous_73418319
- Format
- –
- Schlagworte
- AIDS/HIV, Animals, Base Sequence, Biological and medical sciences, Blotting, Southern, Bronchoalveolar Lavage Fluid, DNA amplification, DNA, Fungal - genetics, DNA, Ribosomal - genetics, HIV Infections - complications, Humans, Infectious diseases, Medical sciences, Molecular Sequence Data, Oligodeoxyribonucleotides, oligonucleotide probe, Oligonucleotide Probes, Parasitic diseases, Pneumocystis - genetics, Pneumocystis - isolation & purification, Pneumocystis carinii, Pneumonia, Pneumocystis - complications, Pneumonia, Pneumocystis - diagnosis, Polymerase Chain Reaction - methods, Rats, RNA, Ribosomal, 16S - genetics, Sequence Alignment, Sequence Homology, Nucleic Acid, Species Specificity, Templates, Genetic