Nature methods, 2009-11, Vol.6 (11), p.805-808
2009
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- Autor(en) / Beteiligte
- Titel
- A chemical platform for improved induction of human iPSCs
- Ist Teil von
- Nature methods, 2009-11, Vol.6 (11), p.805-808
- Ort / Verlag
- United States: Nature Publishing Group
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1548-7091eISSN: 1548-7105DOI: 10.1038/nmeth.1393Titel-ID: cdi_proquest_miscellaneous_734116216
- Format
- –
- Schlagworte
- Benzamides - pharmacology, Biochemistry, Cell Differentiation - genetics, Dioxoles - pharmacology, Diphenylamine - analogs & derivatives, Diphenylamine - pharmacology, Efficiency, Fibroblasts - physiology, Humans, Induced Pluripotent Stem Cells - cytology, Induced Pluripotent Stem Cells - physiology, Kinetics, MAP Kinase Kinase 1 - antagonists & inhibitors, Pyrimidines - pharmacology, Receptors, Transforming Growth Factor beta - antagonists & inhibitors, Research methodology, Stem cells, Thiazoles - pharmacology, Transduction, Genetic