Details

Autor(en) / Beteiligte

• Oh, D.-Y
• Schumann, R.R
• Hamann, L
• Neumann, K
• Worm, M
• Heine, G

Titel

Association of the toll-like receptor 2 A-16934T promoter polymorphism with severe atopic dermatitis

Ist Teil von

• Allergy (Copenhagen), 2009-11, Vol.64 (11), p.1608-1615

Ort / Verlag

Oxford, UK: Oxford, UK : Blackwell Publishing Ltd

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Atopic dermatitis (AD) is a chronic inflammatory skin disease with a multifactorial pathogenesis and increasing incidence in the Western world. A genetically determined defective function of pattern recognition receptors such as toll-like receptors (TLRs) has been proposed as a candidate mechanism in the pathogenesis of AD. To study the impact of genetic predisposition of five genes encoding for pattern recognition-related molecules for the phenotype of AD. We examined nine different single-nucleotide polymorphism (SNP) frequencies in the genes encoding TLR1, -2, -4, -9 and the adapter molecule TIRAP by PCR with subsequent melting curve analysis in a case/control cohort of 136 adult AD patients and 129 age and gender matched non-atopic, healthy individuals. TLR2-expression and -function in cells from genotyped individuals were analysed. For the SNPs examined, similar genotype frequencies were found in both groups. In a subgroup of patients suffering from severe AD (SCORAD >50), a significantly increased representation of the A-allele in position -16934 of the tlr2 gene was present (P = 0.004). Constitutive tlr2 mRNA expression in peripheral monocytes was independent of this tlr2 promoter SNP. Stimulation assays indicated that IL-6, but not TNF-α secretion following TLR2 stimulation is reduced in homozygous tlr2-16934-A allele carriers. These data indicate that TLR2 is relevant for the phenotype of severe AD in adults.