Details

Autor(en) / Beteiligte

• Fassmann, Antonin
• Holla, Lydie Izakovicova
• Buckova, Dana
• Vasku, Anna
• Znojil, Vladimir
• Vanek, Jiri

Titel

Polymorphisms in the +252(A/G) lymphotoxin-alpha and the −308(A/G) tumor necrosis factor-alpha genes and susceptibility to chronic periodontitis in a Czech population

Ist Teil von

• Journal of periodontal research, 2003-08, Vol.38 (4), p.394-399

Ort / Verlag

Oxford, UK: Munksgaard International Publishers

Erscheinungsjahr

2003

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Background:  Periodontitis is an inflammatory disease that leads to irreversible attachment loss, bone destruction and eventually tooth loss. Tumor necrosis factor (TNF), a pluripotent proinflammatory cytokine that is able to induce tissue destruction and bone resorption, has been implicated in the pathogenesis of periodontal disease. Methods:  In this study, we investigated an association between chronic periodontitis and two previously described bi‐allelic polymorphisms in the TNF locus: a G to A transition at position −308 in the 5′promoter region of the TNF‐α gene and an NcoI restriction fragment length polymorphism (RFLP) in the first intron (position +252A/G) of the lymphotoxin α (LT‐α) gene. Genomic DNA was obtained from 132 patients with chronic periodontitis together with 114 age‐ and gender‐matched unrelated control subjects. Results:  The TNF‐α (−308G/A) polymorphism itself showed no association with chronic periodontitis, whereas the frequency distribution of the LT‐α (+252A/G) genotypes showed statistically significant differences between patients and the reference group. The proportion of individuals carrying the LT‐α 1/1 genotype was significantly lower in the group of patients with chronic periodontitis (0.8%) than in the control group (8.8%) (P < 0.0094, Pcorr < 0.05). However, the significant differences in the frequencies of the combined genotypes (TNF‐α and LT‐α) between the control and the patient groups were found using a simulation by applying the Monte‐Carlo method (P < 0.01). Conclusion:  Our data suggest that combined genotypes composed of the TNF‐α and LT‐α gene polymorphisms may influence the susceptibility to chronic periodontitis. We also showed that, comparing the two genes, the 1/1 genotype of the NcoI polymorphism in the first intron of the LT‐α gene is a more informative marker and it may be one of the protective genetic factors against chronic periodontitis in our population.