Details

Autor(en) / Beteiligte

• Suzuki, Ryota
• Ihira, Masaru
• Enomoto, Yoshihiko
• Yano, Hiroaki
• Maruyama, Fumio
• Emi, Nobuhiko
• Asano, Yoshizo
• Yoshikawa, Tetsushi

Titel

Heat denaturation increases the sensitivity of the cytomegalovirus loop-mediated isothermal amplification method

Ist Teil von

• Microbiology and immunology, 2010-08, Vol.54 (8), p.466-470

Ort / Verlag

Melbourne, Australia: Blackwell Publishing Asia

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• ABSTRACT A new protocol for CMV LAMP with an additional heat denaturation step was developed. While the sensitivity of the original CMV LAMP method was 500 copies/tube, sensitivity was increased by up to 100 copies/tube by additional heat denaturation. CMV DNA was detected in 103 of 350 samples (29.4%) by the original CMV LAMP procedure and 148 of 350 samples (42.3%) by the new CMV LAMP protocol. When the pp65 antigenemia assay was used as the standard method, the sensitivity, specificity, PPV, and NPV of the new protocol were 92.9%, 77.7%, 62.2%, and 96.5%, respectively.