Details

Autor(en) / Beteiligte

• Lai, Chih-Ho
• Chang, Nai-Wen
• Lin, Chin-Fen
• Lin, Chia-Der
• Lin, Ying-Ju
• Wan, Lei
• Sheu, Jim Jinn-Chyuan
• Chen, Shih-Yin
• Huang, Yi-Ping
• Sing, Yu-Ting
• Tao, Tse-Wen
• Lai, Cheng-Kuo
• Tsai, Ming-Hsui
• Chan, Hong-Lin
• Jou, Yu-Jen
• Lin, Cheng-Wen

Titel

Proteomics-based identification of haptoglobin as a novel plasma biomarker in oral squamous cell carcinoma

Ist Teil von

• Clinica chimica acta, 2010-07, Vol.411 (13), p.984-991

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• Identification of tumor biomarkers to assist early diagnosis and monitoring of disease progression may potentially decrease the mortality and morbidity associated with oral cancer. A mouse model with oral squamous cell carcinoma (OSCC) induced by 4-nitroquinoline 1-oxide (4-NQO)/arecoline in drinking water was established to discover stage-associated biomarkers. A proteomics approach, immunoblot and immunohistochemical analysis were used to validate the expressed biomarkers in mice with OSCC. Human plasma samples were also collected and candidate biomarkers were evaluated using enzyme-linked immunosorbent assay. Proteomic profiling of mouse plasma samples indicated that haptoglobin and apolipoprotein A1 precursor were up-regulated in the mice with OSCC. Immunoblotting of plasma samples and immunohistochemical analysis of oral tissues showed a significantly higher level of haptoglobin in the OSCC mice than in the control mice. The expression of haptoglobin in human plasma samples from 52 patients with OSCC indicated a strong correlation between the increasing levels of haptoglobin and the clinical stages of OSCC ( P < 0.01). These results suggest that haptoglobin has a great potential as a sensitive plasma biomarker for early detection of patients with OSCC.