Details

Autor(en) / Beteiligte

• Albarbarawi, Osama
• Barton, Alun
• Lin, ZhaoSheng
• Takahashi, Eddie
• Buddharaju, Ajay
• Brady, Jeffrey
• Miller, Douglas
• Palmer, Colin N. A
• Huang, Jeffrey T.-J

Titel

Measurement of Urinary Total Desmosine and Isodesmosine Using Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry

Ist Teil von

• Analytical chemistry (Washington), 2010-05, Vol.82 (9), p.3745-3750

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• The current LC-MS based desmosine/isodesmosine (DES/IDS) assays may be unsatisfactory for clinical use due to lack of an appropriate internal standard or low throughput. A fast and reliable LC-MS method using a D5-DES as an internal standard for measuring urinary total DES/IDS was developed and validated in this study. The reportable range of this assay was 1.0 and 480.0 ng/mL. The intra- and interassay imprecision, accuracy, and recovery for quality control samples were within acceptable range (<25%). Urinary total DES/IDS level was stable at room temperature or 4 °C for 20 h, and for three freeze/thaw cycles. The assay was employed to measure urine samples from COPD patients and demographically matched healthy volunteers. The total urinary DES/IDS levels were approximately 3-fold higher in COPD patients compared to healthy volunteers. The suitability of using urinary free DES to estimate elastin degradation was also evaluated in a second cohort. Despite urinary free and total DES/IDS levels being highly correlated, our data suggest that urinary total DES/IDS level is a preferred biomarker for elastin degradation. These results demonstrate that the LC-MS/MS method provides sensitive, reproducible and accurate quantification of urinary total DES/IDS as a biomarker for monitoring elastin degradation in diseases such as COPD.