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Proteomic analysis of early-response to mechanical stress in neonatal rat mandibular condylar chondrocytes
Journal of cellular physiology, 2010-06, Vol.223 (3), p.610-622
Li, Huang
Yang, Han-Shuo
Wu, Tuo-Jiang
Zhang, Xiang-Yu
Jiang, Wen-Hui
Ma, Qiao-Lin
Chen, Yang-Xi
Xu, Yun
Li, Song
Hua, Zi-Chun
2010
Details
Autor(en) / Beteiligte
Li, Huang
Yang, Han-Shuo
Wu, Tuo-Jiang
Zhang, Xiang-Yu
Jiang, Wen-Hui
Ma, Qiao-Lin
Chen, Yang-Xi
Xu, Yun
Li, Song
Hua, Zi-Chun
Titel
Proteomic analysis of early-response to mechanical stress in neonatal rat mandibular condylar chondrocytes
Ist Teil von
Journal of cellular physiology, 2010-06, Vol.223 (3), p.610-622
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2010
Link zum Volltext
Quelle
Wiley Online Library Journals Frontfile Complete
Beschreibungen/Notizen
The objectives of this study were to investigate the early response to mechanical stress in neonatal rat mandibular chondrocytes by proteomic analysis. To evaluate its molecular mechanism, chondrocytes were isolated and cultured in vitro, then loaded mechanical stress by four‐point bending system on different patterns. Morphological observation, flow cytometric analysis, and MTT assays indicated that 4,000 µstrain loading for 60 min was an appropriate mechanical stimulus for the following proteome analysis, which produced a transient but obvious inhibitory effect on the cell cycle. Therefore, we took a proteomic approach to identify significantly differential expression proteins in chondrocytes under this mechanical stress. Using 2‐DE and MALDI‐TOF, we identified seven differentially expressed proteins including the MAPK pathway inhibitor RKIP, cytoskeleton proteins, actin and vimentin, and other selected proteins. Some differentially expressed proteins were validated by both Western blot analysis and fluorescent staining of cytoskeleton at different loading times. The vimentin and RKIP responsive expression were also proven in vivo in oral orthopedic treatment rats, which was in line with the result in vitro. The histological changes in cartilage also showed the inhibition effect. Furthermore, the expressional level of phosphorylated ERK was increased, which demonstrates the changes in MAPK activity. Taken together, these data indicate that mechanical stress resulted in vimentin expression changes first and then led to the subsequent changes in actin expression, MAPK pathway regulated by RKIP and heat shock protein GRP75. All those changes contributed to the cytoskeleton remolding and cell cycle inhibition, finally led to condylar remodeling. J. Cell. Physiol. 223:610–622, 2010. © 2010 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0021-9541
eISSN: 1097-4652
DOI: 10.1002/jcp.22052
Titel-ID: cdi_proquest_miscellaneous_733860248
Format
–
Schlagworte
Actins - metabolism
,
Animals
,
Animals, Newborn
,
Blotting, Western
,
Cell Cycle
,
Cell Shape
,
Chondrocytes - cytology
,
Chondrocytes - metabolism
,
Chondrocytes - ultrastructure
,
Cytoskeleton - metabolism
,
Extracellular Signal-Regulated MAP Kinases - metabolism
,
Immunohistochemistry
,
Mandibular Condyle - cytology
,
Mass Spectrometry
,
Models, Biological
,
Proteomics - methods
,
Rats
,
Rats, Sprague-Dawley
,
Stress, Mechanical
,
Vimentin - metabolism
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