Trends in biotechnology (Regular ed.), 2010-01, Vol.28 (1), p.46-54
2010
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Details
- Autor(en) / Beteiligte
- Titel
- Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
- Ist Teil von
- Trends in biotechnology (Regular ed.), 2010-01, Vol.28 (1), p.46-54
- Ort / Verlag
- Cambridge, MA: Elsevier Ltd
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The availability of tailored enzymes is crucial for the implementation of biocatalysis in organic chemistry. Enantioselectivity is one key parameter defining the usefulness of an enzyme and, therefore, the competitiveness of the corresponding industrial process. Hence, identification of enzymes with high enantioselectivity in the desired transformation is important. Currently, this is achieved by screening collections and libraries comprising natural or man-made diversity for the desired trait. Recently, a variety of improved methods have been developed to generate and screen this diversity more efficiently. Here, we present and discuss the most important advances in both library generation and screening. We also evaluate future trends, such as moving from random evolution to more rational.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0167-7799eISSN: 1879-3096DOI: 10.1016/j.tibtech.2009.10.001Titel-ID: cdi_proquest_miscellaneous_733700331
- Format
- –
- Schlagworte
- Algorithms, Amino Acid Sequence, Amino acids, Biocatalysis, Biocatalysts, Bioconversions. Hemisynthesis, Biological and medical sciences, Biotechnology, Cell growth, Design, Directed Molecular Evolution - methods, Enzymes, Enzymes - biosynthesis, Enzymes - genetics, Fundamental and applied biological sciences. Psychology, Gene Library, Internal Medicine, Libraries, Methods, Methods. Procedures. Technologies, Multiculturalism & pluralism, Mutagenesis, Mutation, Peptide Library, Protein Engineering - methods, Proteins, Spectrum analysis, Stereoisomerism