Details

Autor(en) / Beteiligte

• Desmarchelier, Aurelien
• Oberson, Jean-Marie
• Tella, Patricia
• Gremaud, Eric
• Seefelder, Walburga
• Mottier, Pascal

Titel

Development and Comparison of Two Multiresidue Methods for the Analysis of 17 Mycotoxins in Cereals by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry

Ist Teil von

• Journal of agricultural and food chemistry, 2010-07, Vol.58 (13), p.7510-7519

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• Two multiresidue methods based on different extraction procedures have been developed and compared for the liquid chromatography electrospray ionization tandem mass spectrometry analysis of 17 mycotoxins including ochratoxin A, aflatoxins (B1, B2, G1, and G2), zearalenone, fumonisins (B1 and B2), T-2 toxin, HT-2 toxin, nivalenol, deoxynivalenol, 3- and 15-acetyldeoxynivalenol, fusarenon-X, diacetoxyscirpenol, and neosolaniol in cereal-based commodities. The extraction procedures considered were a QuEChERS-like method and one using accelerated solvent extraction (ASE). Both extraction procedures gave similar performances in terms of linearity (r 2 > 0.98) and precision (both RSDr and RSDiR < 20%). Trueness was evaluated through participation in four proficiency tests and by the analysis of two certified reference materials and one quality control material. Satisfactory Z scores (|Z| < 2) and trueness values (73−130%) were obtained by the proposed procedures. Limits of quantification were similar by both methods and were within the 1.0−2.0 μg/kg range for aflatoxins, 0.5 μg/kg for ochratoxin A, and the 5−100 μg/kg range for all other mycotoxins tested. The QuEChERS-like method was found to be easier to handle and allowed a higher sample throughput as compared to the ASE method.