Proteins, structure, function, and bioinformatics, 2003-06, Vol.51 (4), p.544-551
2003
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Details
- Autor(en) / Beteiligte
- Titel
- Insights into nonspecific binding of homeodomains from a structure of MATalpha2 bound to DNA
- Ist Teil von
- Proteins, structure, function, and bioinformatics, 2003-06, Vol.51 (4), p.544-551
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2003
- Quelle
- Wiley Online Library - AutoHoldings Journals
- Beschreibungen/Notizen
- The 2.1-A resolution crystal structure of the MATalpha2 homeodomain bound to DNA reveals the unexpected presence of two nonspecifically bound alpha2 homeodomains, in addition to the two alpha2 homeodomains bound to canonical alpha2 binding sites. One of the extra homeodomains makes few base-specific contacts, while the other extra homeodomain binds to DNA in a previously unobserved manner. This unusually bound homeodomain is rotated on the DNA, making possible major groove contacts by side-chains that normally do not contact the DNA. This alternate docking may represent one way in which homeodomains sample nonspecific DNA sequences.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1097-0134Titel-ID: cdi_proquest_miscellaneous_73362696
- Format
- –
- Schlagworte
- Amino Acid Sequence, Base Sequence, Binding Sites - genetics, Crystallography, X-Ray, DNA - chemistry, DNA - genetics, DNA - metabolism, DNA-Binding Proteins - chemistry, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, Fungal Proteins - chemistry, Fungal Proteins - genetics, Fungal Proteins - metabolism, Homeodomain Proteins - chemistry, Homeodomain Proteins - genetics, Homeodomain Proteins - metabolism, Hydrogen Bonding, Models, Molecular, Molecular Sequence Data, Nucleic Acid Conformation, Protein Binding, Protein Conformation, Repressor Proteins - chemistry, Repressor Proteins - genetics, Repressor Proteins - metabolism, Sequence Homology, Amino Acid