American journal of hematology, 2010-05, Vol.85 (5), p.331-339
2010
Details
- Autor(en) / Beteiligte
- Titel
- Differential expression of specific microRNA and their targets in acute myeloid leukemia
- Ist Teil von
- American journal of hematology, 2010-05, Vol.85 (5), p.331-339
- Ort / Verlag
- Hoboken: Wiley Subscription Services, Inc., A Wiley Company
- Erscheinungsjahr
- 2010
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Acute myeloid leukemia (AML) the most common acute leukemia in adults is characterized by various cytogenetic and molecular abnormalities. However, the genetic etiology of the disease is not yet fully understood. MicroRNAs (miRNA) are small noncoding RNAs which regulate the expression of target mRNAs both at transcriptional and translational level. In recent years, miRNAs have been identified as a novel mechanism in gene regulation, which show variable expression during myeloid differentiation. We studied miRNA expression of leukemic blasts of 29 cases of newly diagnosed and genetically defined AML using quantitative reverse transcription polymerase chain reaction (RT‐PCR) for 365 human miRNA. We showed that miRNA expression profiling reveals distinctive miRNA signatures that correlate with cytogenetic and molecular subtypes of AML. Specific miRNAs with consolidated role on cell proliferation and differentiation such as miR‐155, miR‐221, let‐7, miR‐126 and miR‐196b appear to be associated with particular subtypes. We observed a significant differentially expressed miRNA profile that characterizes two subgroups of AML with different mechanism of leukemogenesis: core binding factor (CBF) and cytogenetically normal AML with mutations in the genes of NPM1 and FLT3‐ITD. We demonstrated, for the first time, the inverse correlation of expression levels between miRNA and their targets in specific AML genetic groups. We suggest that miRNA deregulation may act as complementary hit in the multisteps mechanism of leukemogenesis offering new therapeutic strategies. Am. J. Hematol. 2010. © 2010 Wiley‐Liss, Inc.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0361-8609eISSN: 1096-8652DOI: 10.1002/ajh.21667Titel-ID: cdi_proquest_miscellaneous_733557989
- Format
- –
- Schlagworte
- Adult, Aged, Aged, 80 and over, Biological and medical sciences, Case-Control Studies, Cell Differentiation - genetics, Cell Proliferation, Core Binding Factors - physiology, Down-Regulation, Female, fms-Like Tyrosine Kinase 3 - genetics, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Granulocyte Precursor Cells - metabolism, Hematologic and hematopoietic diseases, Humans, Leukemia, Myeloid, Acute - classification, Leukemia, Myeloid, Acute - genetics, Leukemia, Myeloid, Acute - physiopathology, Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis, Male, Medical sciences, MicroRNAs - genetics, MicroRNAs - metabolism, Middle Aged, Mutation, Nuclear Proteins - genetics, Reverse Transcriptase Polymerase Chain Reaction, Up-Regulation, Young Adult