Details

Autor(en) / Beteiligte

• Beers, E.P
• Duke, S.H

Titel

Localization of alpha-amylase in the apoplast of pea (Pisum sativum L.) stems

Ist Teil von

• Plant physiology (Bethesda), 1988-08, Vol.87 (4), p.799-802

Ort / Verlag

Rockville, MD: American Society of Plant Physiologists

Erscheinungsjahr

1988

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Most of the activity of an α-amylase present in crude pea (Pisum sativum L. cv Laxton's Progress No. 9) leaf preparations cannot be found in isolated pea leaf protoplasts. The same extrachloroplastic α-amylase is present in pea stems, representing approximately 6% of total stem amylolytic activity and virtually all of the α-amylase activity. By a simple infiltration-extraction procedure, the majority (87%) of this α-amylase activity was recovered from the pea stem apoplast without significantly disrupting the symplastic component of the tissue. Only 3% of the β-amylase activity and less than 2% of other cellular marker enzymes were removed during infiltration-extraction.