Plant physiology (Bethesda), 1984-05, Vol.75 (1), p.278-280
1984
Details
- Autor(en) / Beteiligte
- Titel
- Electrophoretic Transfer as a Technique for the Detection and Identification of Plant Amylolytic Enzymes in Polyacrylamide Gels
- Ist Teil von
- Plant physiology (Bethesda), 1984-05, Vol.75 (1), p.278-280
- Ort / Verlag
- Rockville, MD: American Society of Plant Physiologists
- Erscheinungsjahr
- 1984
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, α-amylase, and β-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels. Each amylolytic enzyme degrades starch in the transfer gel to its characteristic limit dextrin as it moves through the gel. Various limit dextrins in the starch gel are identified by their characteristic color development in KI/I solution. Isozymes of starch debranching enzyme, α-amylase, and β-amylase can be easily identified in the same gel.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0032-0889eISSN: 1532-2548DOI: 10.1104/pp.75.1.278Titel-ID: cdi_proquest_miscellaneous_733487867
- Format
- –
- Schlagworte
- Alfalfa, alpha -amylase, Analytical, structural and metabolic biochemistry, beta -amylase, Biological and medical sciences, Colors, Dextrins, Electrophoresis, Enzymes, Enzymes and enzyme inhibitors, Fundamental and applied biological sciences. Psychology, Gels, Hydrolases, Hydrolysis, Medicago sativa, Nodules, Short Communications, Starches, Sweet potatoes