Details

Autor(en) / Beteiligte

• Oliveira, Rômulo Tadeu Dias de
• Mamoni, Ronei Luciano
• Souza, José Roberto Matos
• Fernandes, Juliano Lara
• Rios, Francisco José O
• Gidlund, Magnus
• Coelho, Otávio Rizzi
• Blotta, Maria Heloisa Souza Lima

Titel

Differential expression of cytokines, chemokines and chemokine receptors in patients with coronary artery disease

Ist Teil von

• International journal of cardiology, 2009-07, Vol.136 (1), p.17-26

Ort / Verlag

Shannon: Elsevier Ireland Ltd

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Abstract Monocytes/macrophages and lymphocytes have a key role in the pathogenesis of atherosclerosis through the production of inflammatory and anti-inflammatory cytokines. We evaluated mRNA expression and protein production of CCL2, CXCL8, CXCL9, CXCL10, IFN-γ and IL-10 in vitro as well as the expression of the CCR2 and CXCR3 receptors in peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD) and healthy controls in the presence or absence of oxidized LDL (oxLDL). Patients with CAD showed higher constitutive expression of CCL2, CXCL8, CXCL9, CXCL10 and IFN-γ mRNA and, after stimulation with oxLDL, higher expression of CCL2 and CXCL8 mRNA than the control group. We also detected higher levels of CCL2 and CXCL8 in supernatants of oxLDL-stimulated PBMCs from CAD patients than in corresponding supernatants from controls. Patients with CAD had a higher percentage of constitutive CCR2+ and CXCR3+ cells after stimulation with oxLDL. Among CAD patients, the main differences between the stable (SA) and unstable angina (UA) groups were lower IL-10 mRNA production in the latter group. Altogether, our data suggest that PBMCs from CAD patients are able to produce higher concentrations of chemokines and cytokines involved in the regulation of monocyte and lymphocyte migration and retention in atherosclerotic lesions.