Chung-hua fu chʿan kʿo tsa chih, 2009-03, Vol.44 (3), p.209-213
2009
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Details
- Autor(en) / Beteiligte
- Titel
- Analysis different transcriptional factors in different phenotype endometrial cancer cells
- Ist Teil von
- Chung-hua fu chʿan kʿo tsa chih, 2009-03, Vol.44 (3), p.209-213
- Ort / Verlag
- China
- Erscheinungsjahr
- 2009
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- To analysis the activity of transcriptional factors in endometrial cancer cell lines with different estrogen receptor subtypes. The mRNA levels of estrogen receptor (ER) was detected by quantitative RT-PCR, and the activity of transcriptional factors was also analysed by 345-channel protein/DNA array in RL-952 (the expression status of ERalpha and ERbeta both positive), HEC-1A [ERalpha (+/-), while ERbeta negative] and HEC-1B (ERalpha and ERbeta both negative). The transcription factors of NFkappaBp65 and p38MAPK with different activity were tested by enzyme-linked immunosorbent assay (ELISA) to confirm the results of protein/DNA array. The mRNA levels of ERalpha in RL-952, HEC-1A and HEC-1B were (6780+/-282), (684+/-84) and (168+/-38) copy/ng, respectively. Among 345 candidate transcriptional factors, there were 28 factors associated with ER status. Compared with RL-952 cells, 13 transcriptional activity factors were concomitantly up-regulation, while 15 concomitantly down-regulation in HEC-1A and HEC-1B cel
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0529-567XTitel-ID: cdi_proquest_miscellaneous_733363639
- Format
- –
- Schlagworte
- Cell Line, Tumor, Endometrial Neoplasms - metabolism, Endometrial Neoplasms - pathology, Enzyme-Linked Immunosorbent Assay, Estrogen Receptor alpha - genetics, Estrogen Receptor alpha - physiology, Estrogen Receptor beta - genetics, Estrogen Receptor beta - physiology, Female, Gene Expression Regulation, Neoplastic, Humans, p38 Mitogen-Activated Protein Kinases - metabolism, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - genetics, RNA, Messenger - metabolism, Signal Transduction, Transcription Factor RelA - metabolism, Transcription Factors - metabolism