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Analysis different transcriptional factors in different phenotype endometrial cancer cells
Ist Teil von
Chung-hua fu chʿan kʿo tsa chih, 2009-03, Vol.44 (3), p.209-213
Ort / Verlag
China
Erscheinungsjahr
2009
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
To analysis the activity of transcriptional factors in endometrial cancer cell lines with different estrogen receptor subtypes.
The mRNA levels of estrogen receptor (ER) was detected by quantitative RT-PCR, and the activity of transcriptional factors was also analysed by 345-channel protein/DNA array in RL-952 (the expression status of ERalpha and ERbeta both positive), HEC-1A [ERalpha (+/-), while ERbeta negative] and HEC-1B (ERalpha and ERbeta both negative). The transcription factors of NFkappaBp65 and p38MAPK with different activity were tested by enzyme-linked immunosorbent assay (ELISA) to confirm the results of protein/DNA array.
The mRNA levels of ERalpha in RL-952, HEC-1A and HEC-1B were (6780+/-282), (684+/-84) and (168+/-38) copy/ng, respectively. Among 345 candidate transcriptional factors, there were 28 factors associated with ER status. Compared with RL-952 cells, 13 transcriptional activity factors were concomitantly up-regulation, while 15 concomitantly down-regulation in HEC-1A and HEC-1B cel