Details

Autor(en) / Beteiligte

• Sacheck, Jennifer M
• Milbury, Paul E
• Cannon, Joseph G
• Roubenoff, Ronenn
• Blumberg, Jeffrey B

Titel

Effect of vitamin E and eccentric exercise on selected biomarkers of oxidative stress in young and elderly men

Ist Teil von

• Free radical biology & medicine, 2003-06, Vol.34 (12), p.1575-1588

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2003

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Muscle damage resulting from eccentric exercise provides a useful model of oxyradical-induced injury and can be used to examine age-related responses to oxidative stress. Sixteen young (26.4 ± 3.3 years) and 16 older (71.1 ± 4.0 years) healthy men were randomly assigned to 1000 IU/d vitamin E or placebo for 12 weeks and ran downhill for 45 min at 75% VO 2max, once before and following supplementation. Blood samples were obtained before (baseline) and immediately postexercise (0 h), and at 6, 24, and 72 h postexercise to determine antioxidant status, muscle damage, lipid peroxidation, and DNA damage. Following exercise, young and older men experienced similar increases in serum creatine kinase (CK), F 2α-isoprostanes (iPF 2α; p < .001) and malondialdehyde (MDA; p < .01), although iPF 2α peaked at 72 h postexercise and MDA peaked at 0 h. Oxygen Radical Absorbance Capacity (ORAC) decreased at 72 h ( p < .01) and correlated with the rise in iPF 2α, MDA, and CK in the young men ( p < .05). Leukocyte 8-hydroxy-2′-deoxyguanosine (8-OHdG) was unaffected by exercise. Vitamin E decreased peak CK in young men, while in older men it decreased resting levels of iPF 2α and suppressed the 24 h postexercise increases in iPF 2α ( p < .05). Thus, vitamin E supplementation induced modest changes eccentric exercise-induced oxidative stress, although differentially between the young and older subjects, while age had no direct influence on these responses among this group of physically fit subjects.