Details

Autor(en) / Beteiligte

• Tsuchiya, Tokuji
• Kameya, Nanako
• Nakamura, Ikuo

Titel

Straight Walk: A modified method of ligation-mediated genome walking for plant species with large genomes

Ist Teil von

• Analytical biochemistry, 2009-05, Vol.388 (1), p.158-160

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Polymerase chain reaction (PCR)-based genome walking techniques are commonly used to clone unknown genomic regions flanking known sequences. However, these methods are typically problematic when applied to highly complex DNA templates isolated from plants with large genomes. Here we describe a reliable and efficient genome walking method that is particularly effective for plants with large genomes. Our ligation-mediated PCR method, Straight Walk, has improved sensitivity and specificity due to optimization of sequences of adaptors and adaptor primers. Successful genome walking in lily, which has one of the largest genomes in plants, indicates that Straight Walk is applicable for most plant species.