Details

Autor(en) / Beteiligte

• Colomb, Tristan
• Kühn, Jonas
• Charrière, Florian
• Depeursinge, Christian
• Marquet, Pierre
• Aspert, Nicolas

Titel

Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Ist Teil von

• Optics express, 2006-05, Vol.14 (10), p.4300-4306

Ort / Verlag

United States

Erscheinungsjahr

2006

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• In this paper we present a new method to achieve quantitative phase contrast imaging in Digital Holographic Microscopy (DHM) that allows to compensate for phase aberrations and image distortion by recording of a single reference hologram.We demonstrate that in particular cases in which the studied specimen does not have abrupt edges, the specimen's hologram itself can be used as reference hologram. We show that image distortion and phase aberrations introduced by a lens ball used as microscope objective are completely suppressed with our method. Finally the concept of self-conjugated reference hologram is applied on a biological sample (Trypanosoma Brucei) to maintain a spatial phase noise level under 3 degrees.