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Two variants of zebrafish p100 are expressed during embryogenesis and regulated by Nodal signaling
Ist Teil von
FEBS letters, 2003-05, Vol.543 (1), p.190-195
Ort / Verlag
England: Elsevier B.V
Erscheinungsjahr
2003
Quelle
The Electronic Journals Library
Beschreibungen/Notizen
Human p100 protein was first identified as a transcriptional coactivator of Epstein–Barr virus nuclear antigen 2, and has been shown to be a coactivator of other cellular transactivators. Its roles in development of vertebrate embryos, however, have not been reported. We have identified a zebrafish ortholog of the human p100 coactivator. The zebrafish
p100 transcript is processed to two alternative variants, long and short forms, referred to as
p100L and
p100S, respectively. Both GFP-p100L and GFP-p100S fusion proteins are located in the cytoplasm of transfected culture cells and microinjected embryonic cells. Analysis of transcripts with Northern blots revealed the presence of
p100L and lower amounts of
p100S mRNAs from the one-cell stage throughout the life cycle. Whole-mount in situ hybridization shows that
p100L and
p100S share the same spatiotemporal expression pattern. Their zygotic expression is initially restricted to axial mesoderm precursors during gastrulation, and then spreads over other tissues during segmentation, and finally is constrained to some internal organs at day 5. We also find that Nodal signaling is essential for the zygotic expression of
p100. These studies pave the way to understanding in depth the role of p100 during vertebrate embryogenesis.