Details

Autor(en) / Beteiligte

• Li, Chun-Shi
• Zhang, Qinggao
• Lee, Kwang-Jae
• Cho, Sung-Won
• Lee, Kee-Myung
• Hahm, Ki-Baik
• Choi, Suck-Chei
• Yun, Ki-Jung
• Chung, Hun-Taeg
• Chae, Soo-Cheon

Titel

Interleukin-27 polymorphisms are associated with inflammatory bowel diseases in a Korean population

Ist Teil von

• Journal of gastroenterology and hepatology, 2009-10, Vol.24 (10), p.1692-1696

Ort / Verlag

Melbourne, Australia: Blackwell Publishing Asia

Erscheinungsjahr

2009

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Background and Aims:  The cytokine interleukin (IL)‐27 is composed of two subunits, Epstein–Barr virus‐induced gene 3 (EBI3) and p28, and IL‐27 is a novel IL‐12 family member that mediates between the innate and adaptive immune systems. We previously identified four polymorphisms in the human IL‐27 gene and we suggested that the polymorphism of IL‐27 is associated with the susceptibility to asthma. IL‐27 transcripts are significantly elevated in active Crohn's disease (CD) but not in ulcerative colitis (UC). To determine whether these IL‐27 single nucleotide polymorphisms are associated with the susceptibility to inflammatory bowel disease (IBD), the genotype and allelic frequencies of the IL‐27 polymorphisms were analyzed between the IBD patients and the healthy controls. Methods:  Genotype analysis of the IL‐27 gene was performed by the single‐base extension (SBE) method. The haplotype frequencies of IL‐27 for multiple loci were estimated using the expectation maximization (EM) algorithm. Results:  The genotype frequencies of the g.‐964A > G polymorphism in the IBD patients were significantly different from those of the healthy control group (P = 0.001). In both the UC and CD patients, the genotype frequencies of the g.‐964A > G polymorphism were also significantly different from the frequencies of the healthy control group (P = 0.009). The frequencies of the AGT and GGT haplotypes were significantly different between the healthy control group and the IBD patient group (P = 0.00004 and 0.021, respectively). Conclusion:  Our results suggest that the g.‐964A > G polymorphism of the IL‐27 gene located on the IBD1 locus might be associated with the susceptibility to IBD.