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The phytopathogenic fungus
Colletotrichum gloeosporioides was analyzed for chitinase activity, the best production occurring at the fourth day. A 43 kDa endochitinase with specific activity of 413 U μg
−1 protein was purified corresponding to a 75% yield. The optima of temperature and pH for the enzyme were 50°C and pH 7.0, respectively. The enzyme showed a high stability at 50°C and pH 7.0. Values of pH from 5.0 up to 7.0 gave, at least, 50% of maximum activity, suggesting a biotechnological application. Further studies are in progress to determine the possible use of this endochitinase in biological control.