Details

Autor(en) / Beteiligte

• Aires, Viviana A.
• Hinsch, Klaus-Dieter
• Mueller-Schloesser, Frank
• Bogner, Katja
• Mueller-Schloesser, Susanne
• Hinsch, Elvira

Titel

In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen

Ist Teil von

• Theriogenology, 2003-07, Vol.60 (2), p.269-279

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• Semen diluents containing egg yolk as a cryoprotectant may pose hygienic risks and are difficult to standardize. Although a new generation of semen diluents free of animal ingredients is available, egg yolk-containing extenders are still widely used for cryopreserving semen. We compared the effects of using different extenders on bovine sperm function in vitro and on fertility in vivo. A soy lecithin extender (SL; AndroMed ®) and an egg yolk-containing (TRIS-EY) extender were tested. No differences ( P>0.05) were detected between the two extenders for sperm–zona pellucida binding capacity (HZI=115±13). Assessment of the inducibility of the acrosome reaction with progesterone showed no differences ( P>0.05) between extenders for live acrosome-reacted sperm (15±2.36 and 14.42±2.02%, respectively, for SL and TRIS-EY). However, post-thaw sperm motility was significantly lower ( P<0.05) when semen was extended in the TRIS-EY diluent. Field trials revealed that nonreturn rates of SL-extended semen showed significantly higher insemination success ( P<0.0001) compared with the nonreturn rates for the TRIS-EY extender (70.45 and 67.85%, respectively). We suggest that consistent with quality standards that should be required for cryoprotectant media and because of the superior quality of the egg yolk-free extender, a defined soybean lecithin-containing diluter might be the better choice as a semen extender in the future.