FEBS letters, 2003-05, Vol.543 (1), p.154-158
2003
Details
- Autor(en) / Beteiligte
- Titel
- The Drosophila homolog of Aut1 is essential for autophagy and development
- Ist Teil von
- FEBS letters, 2003-05, Vol.543 (1), p.154-158
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2003
- Link zum Volltext
- Quelle
- Elsevier ScienceDirect Journals
- Beschreibungen/Notizen
- The Drosophila homolog of yeast Aut1, CG6877/Draut1, is a ubiquitously expressed cytosolic protein. Draut1 loss of function was achieved by expression of an inverted repeat transgene inducing RNA interference. The effect is temperature-dependent and resembles an allelic series as described by Fortier, E. and Belote, J.M. (Genesis 26 (2000) 240–244). Draut1 loss of function larvae are unable to induce autophagy and heterophagy in fat body cells before pupariation and die during metamorphosis. To our knowledge, this is the first report of a multicellular animal lacking the function of a gene participating in the protein conjugation systems of autophagy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/S0014-5793(03)00431-9Titel-ID: cdi_proquest_miscellaneous_73287623
- Format
- –
- Schlagworte
- 20E, 20-hydroxy-ecdysone, Animals, Aut1/Apg3, Autophagy, Autophagy-Related Proteins, CG6877/Draut1, Draut1p, Draut1 protein, Drosophila, Drosophila - embryology, Drosophila - growth & development, Drosophila - physiology, Drosophila Proteins - genetics, Drosophila Proteins - physiology, EIR, expression of an inverted repeat, EST, expressed sequence tag, Fat body, Fat Body - ultrastructure, Larva - ultrastructure, PAGE, polyacrylamide gel electrophoresis, RNA Interference, RNAi, RNA interference, Saccharomyces cerevisiae Proteins - chemistry, Sequence Homology, UAS, upstream activating sequence, Ubiquitin-Conjugating Enzymes