Details

Autor(en) / Beteiligte

• Sington, J D
• Morris, L S
• Nicholson, A G
• Coleman, N

Titel

Assessment of cell cycle state may facilitate the histopathological diagnosis of malignant mesothelioma

Ist Teil von

• Histopathology, 2003-05, Vol.42 (5), p.498-502

Ort / Verlag

Oxford, UK: Blackwell Science Ltd

Erscheinungsjahr

2003

Quelle

Wiley Online Library All Journals

Beschreibungen/Notizen

• Aims:  The differentiation of benign pleural conditions from malignant mesothelioma may be difficult, especially with a small biopsy. We have tested the hypothesis that assessment of the cell cycle status is of value in the histopathological diagnosis of such biopsies, by comparing 33 malignant mesotheliomas with 36 cases of reactive mesothelial hyperplasia and reactive pleural fibrosis. Methods and results:  Biopsies were investigated for proliferative status by immunostaining for a novel antibody, MCM2, all of which showed nuclear expression of MCM2 at higher frequency than Ki67 (P < 0.0001). Counts in areas of maximum tumour staining showed significantly higher labelling indices (LIMax) in epithelioid and sarcomatoid mesotheliomas compared with reactive mesothelial hyperplasia and reactive pleural fibrosis (P < 0.0001 for both). Average counts (LIAve) revealed a significant increase in epithelioid mesothelioma compared with reactive mesothelial hyperplasia (P < 0.0001). Conclusion:  We consider MCM2 to be a useful adjunct in the differential diagnosis of malignant mesothelioma.