Details

Autor(en) / Beteiligte

• Park, In-Kyu
• Yang, Jun
• Jeong, Hwan-Jeong
• Bom, Hee-Seung
• Harada, Ichiro
• Akaike, Toshihiro
• Kim, Su-Il
• Cho, Chong-Su

Titel

Galactosylated chitosan as a synthetic extracellular matrix for hepatocytes attachment

Ist Teil von

• Biomaterials, 2003-06, Vol.24 (13), p.2331-2337

Ort / Verlag

Netherlands: Elsevier Ltd

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• Galactose moiety as the hepatocyte anchorage was covalently coupled with chitosan for the development of synthetic extracellular matrix. Hepatocytes adhesion to galactosylated chitosan (GC)-coated polystyrene (PS) dish became as high as 94.7% after 2 h incubation whereas the hepatocytes adhesion to chitosan-coated PS dish was 69.1%, indication of galactose-specific recognition between GC molecules and asialoglycoprotein receptors of hepatocytes. The DNA synthesis of the hepatocytes adhered to GC-coated dish was increased in the presence of epidermal growth factor (EGF) at low concentration of GC (0.05 μg/ml) whereas the DNA synthesis of the hepatocytes adhered to GC-coated dish was decreased in the presence of EGF at high concentration of GC (5 μg/ml). The spreading shapes of the hepatocytes adhered to the surface in the presence of EGF at low concentration of GC (0.05 μg/ml) were enhanced than in the absence of EGF. The hepatocytes adhered to the surface at high concentration of GC (5 μg/ml) showed round shapes and exhibited many spheroid formation after 24 h in the presence of EGF.