Details

Autor(en) / Beteiligte

• Tran, Simon D
• Pillemer, Stanley R
• Dutra, Amalia
• Barrett, A John
• Brownstein, Michael J
• Key, Sharon
• Pak, Evgenia
• Leakan, Rose Anne
• Kingman, Albert
• Yamada, Kenneth M
• Baum, Bruce J
• Mezey, Eva

Titel

Differentiation of human bone marrow-derived cells into buccal epithelial cells in vivo: a molecular analytical study

Ist Teil von

• The Lancet (British edition), 2003-03, Vol.361 (9363), p.1084-1088

Ort / Verlag

London: Elsevier Ltd

Erscheinungsjahr

2003

Quelle

Psychology & Behavioral Sciences Collection

Beschreibungen/Notizen

• Adult bone marrow-derived (BMD) cells could be used to repair damaged organs and tissues, but the intrinsic plasticity of these cells has been questioned by results of in-vitro studies suggesting that such cells might fuse with other cells giving the appearance of differentiation. We aimed to determine whether fusion events are important in vivo. To test whether BMD cells can colonise an epithelial tissue and differentiate there without fusion, we did in-situ hybridisation with Y and X chromosome probes labelled with 35-sulphur or digoxigenin, or labelled fluorescently. We did immunohistochemistry with anticytokeratin 13 along with fluorescence in-situ hybridisation to identify Y-chromosome positive buccal epithelial cells in cheek scrapings obtained from five females who had received either a bone-marrow transplant or an allogeneic mobilised peripheral-blood progenitor-cell transplant (enriched in CD34+ cells) from male donors. When examined 4–6 years after male-to-female marrow-cell transplantation, all female recipients had Y-chromosome-positive buccal cells (0·8–12·7%). In more than 9700 cells studied, we detected only one XXXY-positive cell (0·01%) and one XXY cell (0·01%), both of which could have arisen when an XY cell fused with an XX cell. Male BMD cells migrate into the cheek and differentiate into epithelial cells, an occurrence that does not depend on fusion of BMD cells to recipient cells. This finding might be an example of transdifferentiation of haemopoietic or stromal progenitor cells. Plasticity of BMD cells could be useful in regenerative medicine.