Analytical biochemistry, 1992-05, Vol.202 (2), p.356-360
1992
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Details
- Autor(en) / Beteiligte
- Titel
- Calibration of a Clark-type oxygen electrode by tyrosinase-catalyzed oxidation of 4- tert-butylcatechol
- Ist Teil von
- Analytical biochemistry, 1992-05, Vol.202 (2), p.356-360
- Ort / Verlag
- San Diego, CA: Elsevier Inc
- Erscheinungsjahr
- 1992
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- A procedure for calibrating a Clark-type oxygen electrode is described. This method is based on the oxidation of 4- tert-butylcatechol (TBC) by O 2 catalyzed by tyrosinase, to yield 4- tert-butyl- o-benzoquinone (TBCQ). This reaction consumes known amounts of oxygen in accordance with the stoichiometry: 2TBC + O 2 → 2TBCQ + 2H 2O and can be used to determine the relation between the oxygen concentration and the oxygen electrode response. TBCQ is very stable in the reaction medium for more than 30 min and shows no significant breakdown, which makes the calibration possible. A kinetic study of the oxidation of 3,4-dihydroxyphenylalanine by tyrosinase using the oxygen electrode is shown to confirm the validity of the calibration method.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0003-2697eISSN: 1096-0309DOI: 10.1016/0003-2697(92)90118-QTitel-ID: cdi_proquest_miscellaneous_73167744
- Format
- –
- Schlagworte
- Biological and medical sciences, Biosensors, Biotechnology, Calibration, Catechols - chemistry, Electrodes - standards, Fundamental and applied biological sciences. Psychology, Methods, Methods. Procedures. Technologies, Monophenol Monooxygenase - chemistry, Oxidation-Reduction, Various methods and equipments