Details

Autor(en) / Beteiligte

• Thomas, W
• Sellwood, R

Titel

Monoclonal antibodies to a 16-kDa antigen of Serpulina (Treponema) hyodysenteriae

Ist Teil von

• Journal of medical microbiology, 1992-09, Vol.37 (3), p.214-220

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

1992

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• AFRC Institute for Animal Health, Compton, Berks RG16 ONN * Correspondence should be sent to Dr R. Sellwood. Received October 30, 1991 Accepted December 24, 1991 Monoclonal antibodies (MAbs) were produced to an outer-envelope preparation from Serpulina ( Treponema ) hyodysenteriae , the aetiological agent of swine dysentery. Three MAbs (isotype IgG1) were obtained. All three recognised a 16-kDa antigen that was common to most strains of S. hyodysenteriae of different serotypes but was absent from non-pathogenic, porcine intestinal spirochaetes. Immunofluorescence and immunogold labelling studies showed that the 16-kDa antigen was exposed on the surface of intact spirochaetes. The MAbs agglutinated freshly grown cultures of spirochaetes and inhibited growth of S. hyodysenteriae strains in vitro .