Details

Autor(en) / Beteiligte

• Woodhead, Andrea P.
• Stoltzman, Carrie A.
• Stay, Barbara

Titel

Allatostatins in the nerves of the antennal pulsatile organ muscle of the cockroach Diploptera punctata

Ist Teil von

• Archives of insect biochemistry and physiology, 1992, Vol.20 (4), p.253-263

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

1992

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The presence of allatostatins in the nerves of the antennal pulsatile organ muscle of the cockroach Diploptera punctata was confirmed by immunocytochemistry, bioassay, and HPLC. Immunocytochemical reactivity with monoclonal antibody against allatostatin I showed strong allatostatin immunoreactivity in the antennal heart nerve which innervates this muscle with varicostities along the muscle fibers and in the insertion of the muscle on the pulsatile ampullae. Bioassay of Sep‐Pak purified muscle extract demonstrated inhibition of juvenile hormone synthesis by corpora allata in vitro. A dose‐response curve showed maximum inhibition of juvenile hormone synthesis was achieved with 10–20 pulsatile organ muscle eq/corpora allata, and 50% inhibition achieved with an estimated 2.6 pulsatile organ muscle eq. Two successive HPLC separations of the Sep‐Pak purified extract yielded bioactive fractions corresponding to the elution times of the five known allatostatins. © 1992 Wiley‐Liss, Inc.