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Expression and purification of mouse TIMP-1 from E. coli
Ist Teil von
FEBS letters, 1992-08, Vol.307 (3), p.375-378
Ort / Verlag
Amsterdam: Elsevier B.V
Erscheinungsjahr
1992
Quelle
MEDLINE
Beschreibungen/Notizen
Tissue inhibitors of metalloproteinases (TIMPs) constitute a family of secreted glycoproteins involved in regulating extracellular matrix degradation in both normal and malignant tissues. We have expressed a cDNA clone of mouse TIMP-1 as a 22-kDa protein with 12 cysteine residues in
E. coli and purified protein that shows inhibitory activity against collagenase following renaturation by chemical means. The low specific activity and circular dichroism measurements suggest, however, that the renaturation of the mouse recombinant (non-glycosylated) protein is not efficient under the conditions we have used, indicative of either thermodynamic instability or the transition to kinetic intermediates which have very low in vitro refolding rates.