FEBS letters, 1992-08, Vol.307 (3), p.375-378
1992
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Expression and purification of mouse TIMP-1 from E. coli
- Ist Teil von
- FEBS letters, 1992-08, Vol.307 (3), p.375-378
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 1992
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Tissue inhibitors of metalloproteinases (TIMPs) constitute a family of secreted glycoproteins involved in regulating extracellular matrix degradation in both normal and malignant tissues. We have expressed a cDNA clone of mouse TIMP-1 as a 22-kDa protein with 12 cysteine residues in E. coli and purified protein that shows inhibitory activity against collagenase following renaturation by chemical means. The low specific activity and circular dichroism measurements suggest, however, that the renaturation of the mouse recombinant (non-glycosylated) protein is not efficient under the conditions we have used, indicative of either thermodynamic instability or the transition to kinetic intermediates which have very low in vitro refolding rates.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/0014-5793(92)80716-TTitel-ID: cdi_proquest_miscellaneous_73113302
- Format
- –
- Schlagworte
- Analytical, structural and metabolic biochemistry, Animals, Biological and medical sciences, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Cloning, Molecular, Collagen, Collagenase, Electrophoresis, Polyacrylamide Gel, Enzymes and enzyme inhibitors, Escherichia coli, Fundamental and applied biological sciences. Psychology, Glycoproteins - chemistry, Glycoproteins - genetics, Glycoproteins - isolation & purification, high performance liquid chromatography: collagenolysis inhibition, HPLC, Hydrolases, Inclusion Bodies - metabolism, IPTG, isopropylthiogalactoside, Metalloendopeptidases - chemistry, Metalloendopeptidases - genetics, Metalloendopeptidases - isolation & purification, Mice, Plasmids, Protein Conformation, Protein refolding, Recombinant Proteins - chemistry, Recombinant Proteins - genetics, Recombinant Proteins - isolation & purification, TFA, Tissue inhibitor of metalloproteinase, Tissue Inhibitor of Metalloproteinases, trifluoroacetic acid