Details

Autor(en) / Beteiligte

• Brown, Scott W
• Meyers, Randall T
• Brennan, Karen M
• Rumble, Julie M
• Narasimhachari, Nedathur
• Perozzi, Edmund F
• Ryan, John J
• Stewart, Jennifer K
• Fischer-Stenger, Krista

Titel

Catecholamines in a macrophage cell line

Ist Teil von

• Journal of neuroimmunology, 2003-02, Vol.135 (1), p.47-55

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• This study provides the first evidence for catecholamine synthesis and release in the RAW264.7 cell line, an important macrophage model. Although catecholamines were low in unstimulated cells, activation with lipopolysaccharide (LPS) induced tyrosine hydroxylase (TH) mRNA and increased extracellular norepinephrine and intracellular dopamine within 48 h. The catecholamine synthesis inhibitor α-methyl- para-tyrosine (α-mpt) decreased extracellular norepinephrine levels, suggesting release and rapid turnover of newly synthesized norepinephrine. High concentrations of dopamine or norepinephrine (≥100 μM) decreased proliferation and increased apoptosis of macrophages. These anti-proliferative effects were prevented by simultaneous treatment with the anti-oxidant ascorbic acid. Pre-incubation with a glutathione synthesis inhibitor ( l-buthionine-[ S, R]-sulfoximine [ l-BSO]) increased sensitivity to catecholamine-stimulated apoptosis, suggesting that glutathione protects macrophages from both endogenous and exogenous catecholamines.