Details

Autor(en) / Beteiligte

• Gutzki, F.-M.
• Tsikas, D.
• Alheid, U.
• Frölich, J. C.

Titel

Determination of endothelium-derived nitrite/nitrate by gas chromatography/tandem mass spectrometry using (15N)NaNO2 as internal standard

Ist Teil von

• Biological Mass Spectrometry, 1992-02, Vol.21 (2), p.97-102

Ort / Verlag

Chichester, UK: John Wiley & Sons, Ltd

Erscheinungsjahr

1992

Quelle

MEDLINE

Beschreibungen/Notizen

• A sensitive gas chromatographic/tandem mass spectrometric method for the determination of nitrite and nitrate in endothelial cell column perfusates was developed. Nitrite/nitrate were converted in the presence of the internal standard (15N)NaNO2 to the corresponding 1‐nitro‐2,4,6‐trimethoxybenzene (NTMB) derivatives which were subsequently analysed by gas chromatography/tandem mass spectrometry, monitoring m/z = 46 and m/z = 47, respectively. The good reproducibility, the wide linear range of the calibration curve from 25 ng ml−1 to 2500 ng ml−1 nitrite and the low detection limit of 200 pg NTMB allowed a sensitive and accurate quantification of nitrite/nitrate in perfusates of endothelial cell columns. Bradykinin had a stimulating effect on human and bovine endothelial cells and led to a threefold increase of nitrite/nitrate in endothelial cell column perfusates compared to those of unstimulated cells.