Details

Autor(en) / Beteiligte

• Hunter, J.E.
• Bernard, A.
• Fuller, B.J.
• McGrath, J.J.
• Shaw, R.W.

Titel

Measurements of the membrane water permeability (Lp) and its temperature dependence (activation energy) in human fresh and failed-to-fertilize oocytes and mouse oocyte

Ist Teil von

• Cryobiology, 1992-04, Vol.29 (2), p.240-249

Ort / Verlag

San Diego, CA: Elsevier Inc

Erscheinungsjahr

1992

Link zum Volltext

Quelle

ScienceDirect

Beschreibungen/Notizen

• The volumetric response of oocytes during rapid alterations of the extracellular osmotic environment were recorded using video microscopy. From these observations, the kinetics of water loss for human and mouse oocytes were determined over the temperature range 37 to 10 °C, including 37, 30, 20, and 10 °C. The changes in diameter of oocytes were measured over a 5-min period and a computer model was used to derive values for membrane water permeability ( L p) and inactive volume ( V b) and to compare the experimental data to the predicted values. The results for the mouse oocyte L p were comparable to values determined by other methods. However the human data, for both failed-to-fertilize and fresh oocytes, have a wide range of values with large standard deviations. The L p values at the various temperatures were used to calculate the Arrhenius activation energy ( E a. An E a value of 9.48 kcal/mol was found for the fresh mouse oocyte, whereas the activation energy for human oocytes was extremely low, 3.73 kcal/mol for fresh oocytes and 1.93 kcal/mol for failed-to-fertilize oocytes.