Details

Autor(en) / Beteiligte

• Stellato, Cristiana
• de Paulis, Amato
• Ciccarelli, Anna
• Cirillo, Raffaele
• Patella, Vincenzo
• Casolaro, Vincenzo
• Marone, Gianni

Titel

Anti-Inflammatory Effect of Cyclosporin A on Human Skin Mast Cells

Ist Teil von

• Journal of investigative dermatology, 1992-05, Vol.98 (5), p.800-804

Ort / Verlag

Danvers, MA: Elsevier Inc

Erscheinungsjahr

1992

Quelle

MEDLINE

Beschreibungen/Notizen

• We have examined the effects of cyclosporin A (CsA) and cyclosporin H (CsH), which bind with different affinity to cyclophilin, to evaluate the role of this protein in the release of preformed (histamine) and de novo synthesized (prostaglandin D2[PGD2]) mediators of inflammatory reactions from human skin mast cells (HSMC). CsA (2.4–800 nM)inhibited (5–60%) histamine release from HSMC challenged with anti-IgE. CsA exerted little, if any, inhibitory effect on histamine release from HSMC challenged with compound A23187 and substance P, whereas it completely suppressed A23187-induced histamine release from human basophils. Inhibition of histamine release from HSMC challenged with anti-IgE was extremely rapid and was not abolished by washing (three times) the cells before anti-IgE challenge. CsA (2.4–800 nM) markedly inhibited (25–70%) the de novo synthesis of PGD2 from HSMC challenged with anti-IgE. CsH, which has an extremely low affinity for cyclophilin, had no effect on skin mast-cell mediator release. These data suggest that CsA is a potent anti-inflammatory agent acting on HSMC, presumably by interacting with cyclophilin.