Details

Autor(en) / Beteiligte

• Naur, Peter
• Hansen, Carsten Hørslev
• Bak, Søren
• Hansen, Bjarne Gram
• Jensen, Niels Bjerg
• Nielsen, Hanne Linde
• Halkier, Barbara Ann

Titel

CYP79B1 from Sinapis alba converts tryptophan to indole-3-acetaldoxime

Ist Teil von

• Archives of biochemistry and biophysics, 2003, Vol.409 (1), p.235-241

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• The cytochrome P450 CYP79B1 from Sinapis alba has been heterologously expressed in Escherichia coli and shown to catalyze the conversion of tryptophan to indole-3-acetaldoxime. Three expression constructs were made, one expressing the native protein and two expressing proteins with different N-terminal modifications. The native construct gave the highest yield as estimated by enzymatic activity per liter of culture. Spheroplasts of E. coli expressing CYP79B1 were reconstituted with the Arabidopsis thaliana NADPH:cytochrome P450 reductase ATR1 heterologously expressed in E. coli to obtain enzymatic activity. This indicates that the E. coli electron-donating system, flavodoxin/flavodoxin reductase, does not support CYP79B1 activity. Recombinant CYP79B1 has a K m for tryptophan of 29±2 μM and a V max of 36.5±0.7 nmol h −1( ml culture ) −1 . The identity at the amino acid level of CYP79B1 is, respectively, 93 and 84% to CYP79B2 and CYP79B3 from A. thaliana, and 96% to CYP79B5 (Accession No. AF453287) from Brassica napus. The CYP79B subfamily of cytochromes P450 is likely to constitute a group of orthologous genes in the biosynthesis of indole glucosinolates.