Details

Autor(en) / Beteiligte

• Musson, Donald G
• Birk, Kimberly L
• Kitchen, Chester J
• Zhang, Jin
• Hsieh, John Y.K
• Fang, Wei
• Majumdar, Anup K
• Rogers, John D

Titel

Assay methodology for the quantitation of unbound ertapenem, a new carbapenem antibiotic, in human plasma

Ist Teil von

• Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2003-01, Vol.783 (1), p.1-9

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• Ertapenem is a new once-a-day antibiotic with excellent coverage of common community gram negative and gram positive aerobes and anaerobes. It demonstrates nonlinear protein binding in human plasma (about 94% bound). An assay for unbound drug was developed to study the pharmacokinetics of unbound ertapenem in plasma. Unbound drug is separated from plasma samples (1.0 ml) by ultrafiltration using a Centrifree ® centrifugal filter device. Ertapenem (vulnerable to hydrolysis of the beta-lactam moiety) is stabilized in the filtrate by adding an equal volume of 0.1 M MES buffer, pH 6.5 and then is analyzed by reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) absorbance detection (300 nm). Non-specific binding to the Centrifree ® device is <3%. A suitable internal standard is not available. The assay is specific and linear over the concentration range of 0.25 to 100 μg/ml in plasma filtrate. The lower limit of quantitation (LLOQ) is 0.25 μg/ml. Intra-day precision is C.V.<10% and accuracy ranges from 97 to 101% of nominal concentration. Inter-day precision and accuracy were determined using quality control samples (QCs) prepared in plasma ultrafiltrate at 0.5, 12 and 80 μg/ml and stored at −70 °C with stabilizer. Inter-day assay accuracy and precision ranged from 100 to 111% of nominal concentration and 1.8 to 5.3% C.V. ( n=40), respectively. The assay has been used to analyze plasma samples from subjects receiving 500 and 2000 mg i.v. doses of ertapenem (30 min infusion).