FEBS letters, 1992-02, Vol.297 (1), p.43-45
1992
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Details
- Autor(en) / Beteiligte
- Titel
- Localization of proteins forming the outer surface of isolated metaphase chromosomes
- Ist Teil von
- FEBS letters, 1992-02, Vol.297 (1), p.43-45
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 1992
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The outer surface of isolated metaphase chromosomes has been investigated by a method of thermally activated tritium labelling. We show that both chromosomal proteins and DNA are tritium-labelled. Fractionation of the chromosomal proteins reveals that scaffold proteins are the most labelled in condensed and EDTA-decondensed chromosomes. Exposition of some scaffold proteins on the outer surface of metaphase chromosomes is suggested.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/0014-5793(92)80323-9Titel-ID: cdi_proquest_miscellaneous_72859406
- Format
- –
- Schlagworte
- Animals, Biological and medical sciences, Chromatin. Chromosome, Chromosomal Proteins, Non-Histone - metabolism, Chromosomes, EDTA, ethylendiamine-tetraacetic acid, Fibroblasts - metabolism, Fundamental and applied biological sciences. Psychology, HEPES, Metaphase, Metaphase chromosome, Mice, Microscopy, Electron, Molecular and cellular biology, Molecular genetics, N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid, Non-histone protein, Scaffold, TCA, topo II, topoisomerase II, trichloroacetic acid, Tritium