Details

Autor(en) / Beteiligte

• Wirleitner, Barbara
• Reider, Daniela
• Ebner, Susanne
• Böck, Günther
• Widner, Bernhard
• Jaeger, Matthias
• Schennach, Harald
• Romani, Nikolaus
• Fuchs, Dietmar

Titel

Monocyte-derived dendritic cells release neopterin

Ist Teil von

• Journal of leukocyte biology, 2002-12, Vol.72 (6), p.1148-1153

Ort / Verlag

United States: Society for Leukocyte Biology

Erscheinungsjahr

2002

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Increased neopterin concentrations in body fluids are found in diseases associated with activated, cell‐mediated immunity including infections, autoimmune diseases, and certain malignancies. Monocytes/macrophages are known to secrete large amounts of neopterin upon stimulation with interferon‐γ (IFN‐γ). Ontogenetically, the major part of dendritic cells (DC) belongs to the myeloid lineage. Therefore, we investigated whether cultured monocyte‐derived DC can elaborate neopterin. Cells were treated with cytokines in the presence or absence of monocyte‐conditioned medium as a maturation stimulus. DC secreted an average 3.5 nmol/l neopterin. In response to IFN‐γ, cells significantly increased their output of neopterin. In distinction to monocytes/macrophages, neopterin production in DC was highly sensitive to IFN‐α and IFN‐β. Further, lipopolysaccharides (LPS) enhanced neopterin synthesis, whereas tumor necrosis factor α, interleukin (IL)‐1β, IL‐2, IL‐10, and IL‐18 were ineffective. Simultaneously, tryptophan degradation by induction of indoleamine (2,3)‐dioxygenase (IDO) was tested in stimulated cells. Our results showed that IFN‐γ as well as LPS are inducers of IDO in DC.