Archives of biochemistry and biophysics, 1992-02, Vol.292 (2), p.534-538
1992
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- Autor(en) / Beteiligte
- Titel
- Identification of an anion-transport ATPase that catalyzes glutathione conjugate-dependent ATP hydrolysis in canalicular plasma membranes from normal rats and rats with conjugated hyperbilirubinemia (GY mutant)
- Ist Teil von
- Archives of biochemistry and biophysics, 1992-02, Vol.292 (2), p.534-538
- Ort / Verlag
- San Diego, CA: Elsevier Inc
- Erscheinungsjahr
- 1992
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- Rat liver canalicular plasma membranes were found to contain a 37-kDa protein that is immunologically cross-reactive with the dinitrophenyl glutathione-stimulated ATPase previously identified in human tissues. The protein, which was partially purified by affinity chromatography, exhibited ATPase activity dependent on dinitrophenyl glutathione, bilirubin ditaurate, and other dianionic compounds. The localization of this protein in the canalicular membrane and its measured enzymatic activity indicate that it is involved in the transport of glutathione derivatives and other dianionic organic compounds. A rat mutant in which the above transport activities are impaired contained the protein in amounts similar to those in a normal control.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0003-9861eISSN: 1096-0384DOI: 10.1016/0003-9861(92)90027-TTitel-ID: cdi_proquest_miscellaneous_72781097
- Format
- –
- Schlagworte
- Adenosine Triphosphatases - isolation & purification, Adenosine Triphosphatases - metabolism, Adenosine Triphosphate - metabolism, adenosinetriphosphatase, Analytical, structural and metabolic biochemistry, Animals, Anion Transport Proteins, ATP, Binding and carrier proteins, Biological and medical sciences, Carrier Proteins - isolation & purification, Carrier Proteins - metabolism, Cell Membrane - enzymology, Chromatography, Affinity, Electrophoresis, Polyacrylamide Gel, Fundamental and applied biological sciences. Psychology, glutathione, Glutathione - pharmacology, hydrolysis, Hyperbilirubinemia - genetics, Hyperbilirubinemia - metabolism, identification, Kinetics, liver, Liver - enzymology, Male, man, Molecular Weight, plasma membranes, Proteins, Rats, Rats, Inbred Strains, Rats, Mutant Strains, Reference Values, requirements