Journal of neurochemistry, 1992-02, Vol.58 (2), p.548-553
1992
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Details
- Autor(en) / Beteiligte
- Titel
- Affinity Purification of Human τ Proteins and the Construction of a Sensitive Sandwich Enzyme‐Linked Immunosorbent Assay for Human τ Detection
- Ist Teil von
- Journal of neurochemistry, 1992-02, Vol.58 (2), p.548-553
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 1992
- Quelle
- Wiley-Blackwell Journals
- Beschreibungen/Notizen
- : Immunoaffinity chromatography with a monoclonal antibody produced against bovine τ protein was used to purify τ proteins from human brain. Fifty grams of brain tissue yielded τ 2 mg of pure τ proteins. The affinity‐purified human τ was used to produce a high‐titered rabbit anti‐human τ serum. The monoclonal anti‐τ antibody and the polyclonal rabbit anti‐τ serum were then used to construct a sandwich enzyme‐linked immunosorbent assay for detection of human τ proteins, with a sensitivity of 1 ng/ml.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-3042eISSN: 1471-4159DOI: 10.1111/j.1471-4159.1992.tb09754.xTitel-ID: cdi_proquest_miscellaneous_72736187
- Format
- –
- Schlagworte
- Affinity purification, Alzheimer's disease, Animals, Antibodies, Monoclonal, Biochemistry and metabolism, Biological and medical sciences, Central nervous system, Chromatography, Affinity, Enzyme-Linked Immunosorbent Assay - methods, Enzyme‐linked immunosorbent assay, Fundamental and applied biological sciences. Psychology, Humans, Immune Sera, Monoclonal antibodies, tau Proteins - isolation & purification, Vertebrates: nervous system and sense organs, τ protein