FEBS letters, 2002-11, Vol.531 (3), p.421-426
2002
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Details
- Autor(en) / Beteiligte
- Titel
- Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death
- Ist Teil von
- FEBS letters, 2002-11, Vol.531 (3), p.421-426
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2002
- Quelle
- Access via Wiley Online Library
- Beschreibungen/Notizen
- Rana catesbeiana ribonuclease (RC-RNase) exerted strong anti-tumor activity and its cytotoxicity was shown to correlate with differentiation stages of three different hepatoma cell lines. In this study, we demonstrate different RC-RNase cytotoxicity in undifferentiated HL-60 cells and in those that had been induced to differentiate by retinoic acid or dimethylsulfoxide. RC-RNase showed cytotoxicity in undifferentiated HL-60 cells, but not in HL-60 cells undergoing terminal differentiation. Furthermore, the caspase-9/caspase-3 pathway was activated when RC-RNase induced death in undifferentiated HL-60 cells and induction of differentiation led to a reversal of the caspase activation pathway.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/S0014-5793(02)03577-9Titel-ID: cdi_proquest_miscellaneous_72696737
- Format
- –
- Schlagworte
- Amphibian Proteins - pharmacology, Animals, Blotting, Western, Caspase, Caspase 3, Caspase 9, Caspases - metabolism, Cell Death - drug effects, Cell Differentiation, Differentiation, DMSO, dimethylsulfoxide, Endoribonucleases - pharmacology, Enzyme Activation, HL-60 cell, HL-60 Cells, Humans, NBT, nitroblue tetrazolium, PARP, poly(ADP-ribose) polymerase, RA, all-trans-retinoic acid, Rana catesbeiana, Rana catesbeiana ribonuclease, RC-RNase, Rana catesbeiana ribonuclease, TPA, 12-O-tetradecanoyl-13-phorbol acetate