Development (Cambridge), 2002-12, Vol.129 (24), p.5839-5846
2002
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- Autor(en) / Beteiligte
- Titel
- A defect in a novel Nek-family kinase causes cystic kidney disease in the mouse and in zebrafish
- Ist Teil von
- Development (Cambridge), 2002-12, Vol.129 (24), p.5839-5846
- Ort / Verlag
- England: The Company of Biologists Limited
- Erscheinungsjahr
- 2002
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The murine autosomal recessive juvenile cystic kidney ( jck ) mutation results in polycystic kidney disease. We have identified in jck mice a mutation in Nek8 , a novel and highly conserved member of the Nek kinase family. In vitro expression of mutated Nek8 results in enlarged, multinucleated cells with an abnormal actin cytoskeleton. To confirm that a defect in the Nek8 gene can cause cystic disease, we performed a cross-species analysis: injection of zebrafish embryos with a morpholino anti-sense oligonucleotide corresponding to the ortholog of Nek8 resulted in the formation of pronephric cysts. These results demonstrate that comparative analysis of gene function in different model systems represents a powerful means to annotate gene function.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0950-1991eISSN: 1477-9129DOI: 10.1242/dev.00173Titel-ID: cdi_proquest_miscellaneous_72671990
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Cell Nucleus - metabolism, Cloning, Molecular, COS Cells, Danio rerio, Databases as Topic, Disease Models, Animal, Freshwater, Genome, juvenile cystic kidney gene, Kidney Diseases, Cystic - genetics, Mice, Mice, Inbred C57BL, Models, Genetic, Molecular Sequence Data, Mutation, Nek8 protein, NIMA-Related Kinases, Oligonucleotides, Antisense - pharmacology, Physical Chromosome Mapping, Protein Kinases, Protein-Serine-Threonine Kinases - genetics, Protein-Serine-Threonine Kinases - physiology, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - metabolism, Time Factors, Zebrafish, Zebrafish Proteins